Description
This Human C1q/TNF-Related Protein 9 (CTRP9) ELISA Kit is intended for quantitative detection of human CTRP9 in cell culture supernates, serum and plasma (heparin). Strip well format. Reagents for up to 96 tests.
This human CTRP9 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CTRP9 has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: Q20-P333) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CTRP9 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Fibronectin amount of sample captured in plate.
The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated detection polyclonal antibody from goat. Expression system for standard: The C1QTNF9 gene is conserved in chimpanzee, Rhesus monkey, and zebrafish. This gene is mapped to 13q12.12. 42 organisms have orthologs with human gene C1QTNF9